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Results/Acceptance Criteria.
During the incubation period examine the medium for macroscopic evidence of microbial growth. If no evidence of growth is found, sample being examined complies with the test for sterility. If the material being tested render the medium turbid so that the presence or absence of microbial growth can not be determined easily by visual examination, 14 days after the beginning of incubation, transfer the portion of the medium (not less than 1ml), to the fresh vessel of the same medium and then incubate the original and transfer vessel for not less than 4 days. If the no evidence of growth is found, sample being xamined comply with the test for sterility.

If the evidence of growth is found, sample being examined does not comply with the test for sterility, unless it can be clearly demonstrated that the test was invalid for causes unrelated to the product to be examined. The test may be considered invalid under the following condition.

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